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Localization and regulation of Sodium/Calcium exchanger (NCX) expression in crayfish Procambarus clarkii during molting and cold acclimation
Author(s) -
Gao Yongping,
Gillen Christopher M.,
Wheatly Michele G.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a281-d
Subject(s) - in situ hybridization , homeostasis , medicine , endocrinology , sodium calcium exchanger , downregulation and upregulation , crayfish , procambarus clarkii , biology , microbiology and biotechnology , kidney , moulting , calcium metabolism , calcium , chemistry , anatomy , messenger rna , intracellular , biochemistry , ecology , gene , larva
NCX (sodium/calcium exchanger) is a bidirectional Ca 2+ transporter that plays a critical role in Ca 2+ homeostasis. The present study employed real‐time PCR and in‐situ hybridization techniques to compare expression and localization of NCX in both epithelial (antennal gland, kidney) and non‐epithelial tissues (axial abdominal) under experimental conditions known to perturb calcium homeostasis in crayfish: natural molting cycle (hormonally mediated) and cold acclimation (hormonally independent). Our results indicated that NCX was ubiquitously distributed in both epithelial and non‐epithelial tissues but that the expression was greater in muscle and lower in epithelium. During pre/postmolt stages, a upregulation of transcription of NCX mRNA was revealed in both tissues as compared with intermolt stage; however the change was more dramatic in the kidney epithelium. Furthermore, the in situ hybridization indicated that the NCX increase during pre/postmolt stages in kidney occurred mainly in the labyrinth and the nephridal canal, which are the regions associated with ion reclamation. In tail muscle, the increased NCX expression during molting was associated primarily with the extensor muscle. In contrast cold acclimation at 4 C for 4 weeks significantly increased the NCX expression levels in all tissues tested and the increased NCX expression was localized again in the ion absorptive regions of the kidney and in the extensor muscles. (Supported by GrantIBN 0445202 to MGW, YG and CMG).