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Determining the estrogen‐sensitivity of the ER‐alpha mRNA half‐life in human breast cell lines
Author(s) -
ErikSoussi Magda,
Ukpeh Ekom,
Dean Diane
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a281-a
Subject(s) - estrogen , messenger rna , estrogen receptor alpha , breast cancer , cell culture , estrogen receptor , cell , alpha (finance) , endocrinology , medicine , cancer research , biology , chemistry , cancer , gene , biochemistry , construct validity , genetics , nursing , patient satisfaction
In two normal estrogen‐responsive tissues mammalian endometrium and fish liver, the ER‐alpha mRNA half‐life increases in response to estrogen treatment. However, Mary Beth Martin and colleagues (1998) found estrogen treatment decreased the ER‐alpha mRNA stability in the MCF7 breast cancer cell line. The goal of this research was to test the estrogen‐sensitivity of the half‐life for the ER‐alpha mRNA in other breast cancer cell lines and in a normal breast epithelial cell line. Thus in addition to MCF7 cells, we tested two estrogen‐responsive breast cancer cell lines, BT474 and T47D, and the normal estrogen‐responsive breast epithelial cell line, HCC1500. 17beta‐estradiol treated (6hrs) cells were harvested after exposure to actinomycin D for various times (0.5 to 6 hours) and the total RNA was isolated using a Qiagen RNeasy kit. The isolated RNA was used in quantitative real time RT‐PCR in order to determine the half‐life of the ER‐alpha mRNA. Preliminary data indicates that ER‐alpha mRNA half‐life decreases similarly in the tested breast cancer cell lines but increases in the normal breast epithelial cell line.