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Toxicity and detection of carboxylated nanodiamonds on human lung epithelial cells
Author(s) -
Chao JuiI,
Liu KuangKai,
Cheng ChiaLiang,
Chang ChiaChing
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a267-a
Subject(s) - a549 cell , nanodiamond , confocal , fluorescence , biophysics , fluorescence microscope , confocal microscopy , cytotoxicity , chemistry , atomic force microscopy , microscope , flow cytometry , viability assay , nanotechnology , microbiology and biotechnology , cell , materials science , in vitro , biochemistry , biology , diamond , pathology , optics , physics , medicine , organic chemistry
Modification on the surface of nanometer‐sized diamond by caboxylation (carboxylated nanodiamond, CND) has been applied for the conjugation of biological molecules such as DNA and protein. In this study, we examined the cytotoxicity and detection of CNDs on the A549 human lung epithelial cells. Treatment with 5‐nm or 100‐nm CNDs (0.1–100 μg/ml) did not markedly reduce the cell viability and altered the protein expression profile in A549 cells. Nevertheless, CNDs were accumulated in A549 cells observed by atomic force microscope and scanning confocal microscope. Both 5‐ and 100‐nm CND particles exhibited the green fluorescence and were ingested into cells. However, the 5‐nm CNDs but not the 100‐nm CNDs were uptake into the nuclei of these cells. Moreover, the fluorescence intensities in A549 cells were found concentration‐dependently to increase after treatment with 5‐nm CNDs (10–100 μg/ml) by using flow cytometer analysis. The fluorescence intensity of 5‐nm CND was higher than 100‐nm CNDs on cells at equal concentration of CND's treatment. As a whole, it is proposed that 5‐nm CND is more useful nanomaterial for further biological applications based on their uptake ability, detectable and little toxic properties on human cells.