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Increased Bioavailability of a Transdermal Application of a Delta Tocopherol Nanoemulsion Preparation
Author(s) -
Kotyla Timothy,
Kuo Fonghsu,
Moolchandani Vikas,
Wilson Thomas,
Nicolosi Robert
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a266-c
Subject(s) - bioavailability , transdermal , microemulsion , chemistry , chromatography , tocopherol , particle size , pharmacology , vitamin e , medicine , antioxidant , biochemistry , pulmonary surfactant
The aim of this study was to compare the transdermal application of a nanoemulsion versus a microemulsion preparation of delta tocopherol as it relates to particle size and bioavailability. Two separate experiments were performed using seven F1B Syrian Golden hamsters, one week apart. The nanoemulsion and microemulsion preparations consisted of canola oil, polysorbate 80, deionized water and delta tocopherol; the only difference between the two preparations was processing of the nanoemulsion with the Microfluidizer® Processor. Both were formulated into a cream and applied to the shaven dorsal area. The particle size of the microemulsion preparation was 2788 nm compared to 65 nm for the nanoemulsion formulation. Two hours post‐application, hamsters that were applied with the nanoemulsion preparation of delta tocopherol had a 36‐fold greater concentration of plasma delta tocopherol compared to the baseline measurement. From the 2 to 3 hour time point, the plasma concentration level had further significantly increased to greater than 68 fold, compared to the baseline measurement, whereas the plasma from hamsters transdermally administered the microemulsion showed less of a significant increase at 2 and 3 hours (9 and 11 fold). The present study suggests that nanoemulsions produced by use of Microfluidizer® Processor significantly increase's the bioavailability of transdermally‐applied delta tocopherol.