Premium
Plasma Amino Acid Analysis by Tandem Mass Spectrometry: A comparison to Amino acid analyzer
Author(s) -
Sokoro AbdulRazaq,
Lehotay Denis C,
Hunter Wayne,
Schneider Diana
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a266-b
Subject(s) - amino acid analysis , chemistry , amino acid , chromatography , derivatization , ninhydrin , tandem mass spectrometry , mass spectrometry , spectrum analyzer , biochemistry , electrical engineering , engineering
Objective: To validate use of tandem mass spectrometry in the analysis of plasma amino acids in comparison to a dedicated amino acid analyzer. Introduction: Plasma amino acid analysis in regularly performed on a dedicated amino acid analyzer that utilizes a ninhydrin based HPLC technique. Sample run times are long with no resolution between citrulline and homocitrulline. Method: 111 plasma samples were obtained on an anonymous basis and were anayzed on the Biochrome® and API2000 tandem mass spectrometry (MS/MS) for Ala, Arg, Cit, Gly, His, Leu, Met, Orn, Phe, Pro, Tyr, Homocit and Val. Sample preparation for MS/MS involved methanolic precipation, derivatization to the respective dibutyryl ester derivatives of the amino acids and detection by MS/MS. Results: All the amino acids showed good correction (r=0.92–0.99) between the two instruments. In addition, MS/MS was able to distinguish between Cit and Homocit, provided greater sensitivity and specificity and faster turn around time(TAT) (total TAT of 30 min). Conclusion: MS/MS can provide equal or even better service in amino acid analysis as is with dedicated amino acid analyzers.