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Interaction of a Copper Cationic Porphyrin with Quadruplex NA
Author(s) -
Butterworth Andrew,
Azam Mahrukh
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a262-c
Subject(s) - porphyrin , chemistry , cationic polymerization , dna , guanine , g quadruplex , copper , metal , derivative (finance) , stereochemistry , duplex (building) , titration , crystallography , photochemistry , inorganic chemistry , polymer chemistry , nucleotide , organic chemistry , biochemistry , financial economics , economics , gene
Interaction of Cu(II)‐ derivative of 5,10,15,20 mesotetrakis(4‐Methyl‐N‐Pyridyl) porphine tosylate (Cu‐TMPyP4) with guanine rich quadruplex DNA(G‐DNA) is investigated in this study. CuTMPyP4 forms a square planar copper porphyrin complex and is known to interact with duplex DNA in a similar fashion to metal free cationic TMPyP4. Interaction of metal free cationic porphyrin with various forms of quadruplex DNA is well studied although its exact mode of interaction with certain forms of G‐DNA is still a question of controversy. We will report the interaction of TMPYP4 and its Cu(II) derivative with various G‐DNA structures formed from telomeric sequences. The binding modes, binding affinities and number of bound ligands per G_DNA molecule will be deduced from Absorption titration curves, scatchard plots, continuous variation analysis etc.