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Microarray Analysis of the Postnatal Conditionally Deleted BDNF Brain
Author(s) -
Makar Tapas,
Trisler David,
Trisler David,
Sultana Shireen,
Bever Christopher
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a252-a
Subject(s) - brain derived neurotrophic factor , neurotrophic factors , neurotrophin , tropomyosin receptor kinase b , biology , medicine , endocrinology , gene knockdown , microarray analysis techniques , cerebral cortex , microbiology and biotechnology , neuroscience , gene , gene expression , receptor , genetics
Brain‐derived neurotrophic factor (BDNF) is involved in brain plasticity and neuronal survival during development. Less is known about the role of BDNF in the adult CNS. To explore the role of this neurotrophin, we have generated conditional mutants in which BDNF has been eliminated from the CNS after birth through the use of the cre‐loxP recombination system. We used BDNF(+/−) mice and wild‐type littermate mice to identify BDNF induced genes in brain cortex of BDNF (+/−) mice using microarray technology, whose expression is altered as a consequence of postnatal knockdown of BDNF. Messenger ribonucleic acid from the brain cortex of BDNF (+/−) and wild type mice were hybridized with microarray membranes. Interestingly, we found certain significant genes such as BDNF gene (2.5 decrease), calcium/calmodium‐dependent protein kinase II alpha (7.2 fold increase), potassium large conductance calcium‐activated channel (3.4 fold increase), GABA‐A receptor, subunit alpha 5 (2.2 fold increase), and acidic (leucine‐rich) nuclear phosphoprotein 32 family (7.1 fold increase). This study demonstrates an involvement of several genes in regulating BDNF function in the adult brain.