Premium
TRAP220 interacts with CCAAT/enhancer‐binding protein‐beta and regulates IFN‐induced gene transcription
Author(s) -
Li Hui,
Gade Padmaja,
Kalvakolanu Dhan V.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a251-c
Subject(s) - transcription factor , mediator , enhancer , ccaat enhancer binding proteins , transcription (linguistics) , microbiology and biotechnology , response element , biology , chemistry , promoter , gene , dna binding protein , gene expression , biochemistry , linguistics , philosophy
Transcription factor CCAAT/enhancer‐binding protein‐β (C/EBP‐β) induces gene transcription through a novel γ‐IFN activated transcriptional element. Thyroid hormone receptor associated protein (TRAP) 220 has been identified as a transcriptional mediator. Our preliminary data has showed that C/EBP‐β significantly induces IRF‐9 promoter in the presence of IFN‐gamma, interestingly, TRAP220 cooperates with C/EBP‐β to induce gene transcription. Co‐immunoprecipitation for in vitro translated proteins and over‐expressed proteins in cells demonstrated a physical association between TRAP220 and C/EBP‐β. The mapping study suggested that N‐terminus (154aa) of TRAP220 contributes this association in vivo. The exact domains or motifs of TRAP220 with which C/EBP‐β interacts and cooperates synergistically are under an active investigation. Thus, TRAP220 interacts with C/EBP‐β and regulates γ‐IFN activated gene transcription. This work was supported by National Institutes of Health Grants CA78282 and CA105005.