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Coning of Putative Zinc Transport Proteins from Chlamydomonas reinhardtii
Author(s) -
Gingrich David J.,
McBride Fancy T.,
Case Mary E.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a245-a
Subject(s) - chlamydomonas reinhardtii , chlamydomonas , cloning (programming) , gene , biology , efflux , genome , cdna library , complementary dna , biochemistry , genetics , mutant , computer science , programming language
In order for cells to regulate intracellular metal ion concentrations, the transport of the ions across cell membranes must be regulated. For zinc ions, these transport proteins may be categorized into two major families, the CDS (ZIP) influx proteins and the ZIP efflux proteins. Chlamydomonas reinhardtii is a unicellular green algae model for eukaryotic plant cells. Analysis of Chlamydomonas genomes has identified several putative metal ion transporters, including those for zinc. We have designed PCR primers based upon the gene sequences for the putative zinc transporters CrMPT1 and CrZIP6 transporters to amply DNA corresponding to the complete gene sequences from a Chlamydomonas reinhardtii cDNA library. PCR products of the appropriate size have been obtained and the sequences are in agreement with the complete sequences predicted from the Chlamydomonas Genome Database. Further cloning into expression vectors is underway. This work is supported by grants from SUNY Potsdam and the State of New York/UUP Professional Development Committee.