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TDP‐43 immunohistochemistry reveals extensive neuritic pathology in FTLD‐U: a Midwest‐Southwest Consortium for FTLD study
Author(s) -
Hatanpaa Kimmo J.,
Bigio Eileen H.,
Cairns Nigel J.,
Schneider Julie A.,
Foong Chan,
Hladik Christa,
Shang Ping,
White Charles L.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a24-c
Subject(s) - frontotemporal lobar degeneration , pathology , neuropathology , senile plaques , autopsy , immunohistochemistry , medicine , hippocampus , alzheimer's disease , biology , neuroscience , frontotemporal dementia , dementia , disease
Recently, TDP‐43 was identified as a component of the inclusions in frontotemporal lobar degeneration with ubiquitinated inclusions (FTLD‐U). Aims : To examine the extent of TDP‐43 pathology in FTLD‐U cases, including cases with sparse or regionally limited ubiquitinated inclusions; To confirm the absence of TDP‐43 pathology in other diseases.Methods : Forty brain autopsy cases of FTLD‐U were obtained from the Midwest‐Southwest Consortium for FTLD. Twenty cases of other neurodegenerative diseases and 5 controls were also studied. Methods: IHC was performed with polyclonal antibodies to TDP‐43 (ProteinTech; 1:1,000) and ubiquitin (Dako; 1:500). Two IHC methods were used: routine and enhanced. The enhanced method included the use of the Benchmark XT automated stainer and ultraView Red detection system (Ventana). Results : A particularly striking pattern of pathology was seen in 6 of 18 cases where the hippocampus was stained with the enhanced method and consisted of frequent dystrophic neurites in the CA1 region. Four of five cases also showed frequent dystrophic neurites in the frontal cortex. No TDP‐43‐pathology was identified in other diseases or controls. Conclusion: Extensive TDP‐43‐immunoreactive neuritic pathology may characterize most cases of FTLD‐U. Support: NACC 2005‐05, NIH AG12300, Winspear Family Center for Research on the Neuropathology of Alzheimer Disease, McCune Foundation