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A study on the phospholipid profile of rat testis and their in vitro deacylation by endogenous phospholipases. A chromatographic and densitometric analysis.
Author(s) -
Helmy Fatma,
Juracka Amal,
Nosavanh LaGina Merie,
Haynes Huldah
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a239
Subject(s) - sphingomyelin , endogeny , phospholipid , cardiolipin , phospholipase , incubation , in vitro , in vivo , chemistry , plasmalogen , phospholipase a2 , ceramide , lipid signaling , biochemistry , enzyme , biology , cholesterol , membrane , apoptosis , microbiology and biotechnology
Several detailed reviews on cellular phospholipase A 2 (cPLA 2 ) have been published, yet the role of cPLA 2 in cell function remains unclear and has made it necessary to continue investigation into this area, in search of full understanding of the in vivo function of all phospholipases endogenous to an organ. Recently, we have reported that the endogenous cardiolipin (CL) of various mammalian myocardia has been shown to be deacylated by an endogenous phospholipase (PLA 1 and/or PLA 2 ), during a 60 minutes in vitro incubation at pH 7.4 and 38 o C of whole tissue homogenate producing monolysocardiolipin (MLCL) and concurrent reduction of CL. In contrast rat spleen treated similarly selectively deacylated ethanolamine plasmalogen (PE) producing lyso PE and concurrent reduction of PE. In the present study, rat testis treated similarly revealed the following results: deacylation of PE producing LPE and concurrent reduction of PE, hydrolysis of sphingomyelin (SM) resulting in ceramide formation and reduction in SM level, and noticeable high level of MLCL and very low level of CL were present in all samples studied (control and incubated testes from one month, 3 months, and 6 months old rats). These data raised some questions pertaining to the lipolytic capabilities of diverse tissues and will be discussed in details.