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Changes in the backbone ceramide subspecies as mouse embryonic stem cells develop into embroid bodies
Author(s) -
Park Hyejung,
Haynes Christopher A.,
Nash Rodney,
Dalton Stephen,
Nairn Alison V.,
Moremen Kelley,
Merrill Alfred H.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a237-a
Subject(s) - sphingolipid , ceramide , sphingomyelin , embryonic stem cell , sphingosine , biochemistry , lipid signaling , chemistry , microbiology and biotechnology , glycosphingolipid , biology , gene , membrane , enzyme , apoptosis , receptor
Sphingolipids (SLs) and glycosphingolipids (GSLs) play significant roles in cell type specific recognition, cell‐cell interaction, cell‐substratum adhesion and other events in embryogenesis. To determine how the amounts and types of sphingolipids change during embryogenesis, liquid chromatography, electrospray ionization tandem mass spectrometry was used to analyze the molecular subspecies of ceramides, sphingomyelins and some glycosphingolipids, related intermediates and signaling molecules (sphingoid bases and 1‐phosphates) as well as key precursors (fatty acyl‐CoA's) of mouse embryonic stem cells (mESCs) and embroid bodies (EB). There were significant increases in very‐long‐chain fatty acids in the ceramide backbone of EB vs ESC that are likely to reflect changes in LASS ( L ongevity ass urance homolog) family ceramide synthases, and perhaps fatty acyl‐CoA elongases, for which there was also a correlate in changes in gene expression pattern. Because the lipid backbones of sphingolipids can influence both membrane structure and signaling, these changes may provide clues about important early developmental events. Supported by NIH grant PA‐02‐132.