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Colchicine causes increased matrix metalloproteinase expression and extracellular matrix degradation in hydra
Author(s) -
Zhang Xiaoming Zhang,
Aufschnaiter Roland,
Li Li,
Athiyal Matthews,
Ren Fengzhen,
Shimizu Hiroshi,
Sarras Michael P
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a234-c
Subject(s) - lernaean hydra , extracellular matrix , matrix metalloproteinase , colchicine , microbiology and biotechnology , laminin , basement membrane , chemistry , matrix (chemical analysis) , integrin , biology , cell , biochemistry , genetics , chromatography
Hydra extracellular matrix (ECM) is located in between two epithelial cell layers, both of which contribute to the synthesis of this matrix. Hydra ECM is one of the early forms of matrix containing a basement membrane and an interstitial matrix. Laminin, type IV collagen and other types of fibrillar collagens are found in hydra ECM. Colchicine inhibits microtubule polymerization and has anti‐mitotic, anti‐inflammatory, and anti‐fibrotic effects. Hydra polyps treated with colchicine reduce or eliminate the fast‐dividing interstitial cells and their derivatives. In addition, we have observed degradation of hydra ECM under 0.4% (10mM) colchicine treatment. The ECM degradation is associated with increased expression of matrix metalloproteinases. Three hydra metalloproteinases, HMP‐1, HMP‐2, and HMMP together with MMP inhibitors are tested. The mechanism connecting microtubule disruption and matrix degradation, which may possibly involve NF‐kB and integrins, are currently investigated.