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Lead hematoxylin‐tartrazine yellow depicts connective tissue changes in rat aorta after balloon angioplasty
Author(s) -
BlandoHoegler Charlene F,
Smith Carolyn J,
Hoegler Carl S
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a231-b
Subject(s) - h&e stain , connective tissue , medicine , vasa vasorum , pathology , anatomy , balloon , staining
Hematoxylin and eosin (H&E) is part of an array of stains traditionally used to highlight the morphology of elastic arteries (Presnell & Schreibman, 1997). In the present study aortic rings of one year old male Sprague‐Dawley rats that had undergone balloon angioplasty (BAL) were stained with H&E and compared with lead hematoxylin‐tartrazine yellow (LH/TY) stained rings. The rings had been harvested at 24 hours, 7 days and 14 days after BAL. In comparison to H&E, the LH/TY stained rings clearly depicted smooth muscle and connective tissue elements in the vessel layers. Twenty‐four hours after BAL, infiltrating macrophages were evident. Microarchitectural changes of all three layers persist for at least 14 days with evidence of neo‐intimal thickening, distension of small blood vessels ( vasa vasorum ), neutrophil migration, cell necrosis and a spongy appearance of the smooth muscle layers. It appears that the LH/TY method more clearly discerns changes in connective tissue in the media during the post‐BAL period. Research support from Keenan Fund of Pace University.