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Characterization of adult neural crest stem cells from human hair follicles
Author(s) -
Krejci Eliska,
Grim Milos
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a229-d
Subject(s) - neural crest , stem cell , biology , stem cell marker , microbiology and biotechnology , amniotic stem cells , nestin , adult stem cell , ectoderm , neurosphere , homeobox protein nanog , neural stem cell , hair follicle , sox10 , embryonic stem cell , pathology , induced pluripotent stem cell , embryo , embryogenesis , medicine , genetics , gene
The neural crest (NC) is a transient embryonic tissue that originates between neural and non‐neural ectoderm. Following neural tube closure, NC cells undergo epithelial‐mesenchymal transition, invade the embryo and differentiate into variety of cell types. Nevertheless, a subpopulation of NCCs retains their stem character. The aim was the isolation and cultivation of adult NC stem cells (NCSCs) from human hair follicles using the method established for murine cells (PMID: 15366003). Follicle explants were prepared from face, pubic or forearm skin. The tissue was obtained from patients undergoing surgery with their consent. Stellate cells emigrated from the explanted follicle after 4–5 days in culture. Immunohistochemistry revealed that these cells expressed NC marker Sox10 and NCSCs marker nestin. After prolonged cultivation the cells differentiated into neurons (beta III tubulin), smooth muscle cells (SMA), glia or melanocyte precursors (S‐100). RT‐PCR technique revealed the expression of further NC markers slug and snail as well as stem cell marker nanog and some differentiation markers of NC progeny. As a conclusion we can say, that human epidermal NCSCs could be a candidate for stem cell therapy, especially due to easily accessible source tissue. Nevertheless targeted differentiation into different NC progeny remains to be tested. Supported by MSMT CR projects 21620806‐3; 1M0021620803