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Differentially expressed genes analysis of mesenchymal stem cells from human umbilical cord blood and bone marrow
Author(s) -
Kim Hyun Jae,
Lee Seung Ku,
Cha YunYi,
Kim Hyoun Geun,
Yun HongShik,
Kang HyunJun,
Kim Kyoung Yeon,
Kwack KyuBum
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a229-a
Subject(s) - mesenchymal stem cell , biology , bone marrow , umbilical cord , stem cell , cord lining , gene , stem cell transplantation for articular cartilage repair , microarray , population , microarray analysis techniques , microbiology and biotechnology , immunology , adult stem cell , gene expression , cellular differentiation , genetics , medicine , environmental health
Mesenchymal stem cells (MSCs) retain both self‐renewal and multilineage differentiation capabilities. In addition, MSCs can be obtained from many sites in the adult or the fetus. Despite wide therapeutic potential, many aspects of human MSCs, particularly the molecular parameters to define the stemness, remain largely unknown. Using high‐density oligonucleotide microarrays, we obtained the differential gene expression profile between a fraction of mesenchymal stem cells from human umbilical cord blood (UCB) and bone marrow (BM). We isolate MSCs from both umbilical cord blood and bone marrow that are morphologically and immunophenotpically. In culture, these two population cells are capable of differentiating in vitro into adipocytes, osteoblasts, and condrocytes. The gene expression profiles of BM‐MSCs and of UCB‐MSCs were compared by oligonucleotide microarray analysis. The two lineages shared most of expressed transcripts that are composed 75% of all the genes number with statistical significance in replicated microarray data (10,777 genes). However differentially expressed genes in both differently originated cells are large portion; number of highly expressed genes in BM‐MSC are 1,306 and in UBC‐MSC are 1,387. According to these results, common characters of UCB‐ and BM‐MSCs can be defined by molecular signature. However, some differentilly expressed genes in each originated MSC are associated with a little differences that are self‐renawal capacity and multipotency.