Premium
Amniotic Fluid Derived Stem Cells for Cardiac Therapeutics
Author(s) -
Delo Dawn,
Neshat Samira,
Christ George,
Groban Leanne,
Olson John,
Zhu JianMing,
Atala Anthony,
Soker Shay
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a229
Subject(s) - amniotic fluid , amniotic stem cells , stem cell , myocyte , regeneration (biology) , microbiology and biotechnology , cell therapy , cardiac muscle , cardiac function curve , troponin i , chemistry , biology , adult stem cell , medicine , anatomy , fetus , endothelial stem cell , in vitro , heart failure , myocardial infarction , biochemistry , pregnancy , genetics
Cell therapy has been proposed as a means to promote the regeneration of injured heart muscle. We have established lines of broad‐spectrum multipotent stem cells derived from primitive fetal cells present in human and mouse amniotic fluid (AFS) cells. We hypothesized that AFS cells can be used for cardiac tissue regeneration. AFS cells were induced to cardiogenic differentiation, seeded on scaffolds and preconditioned in a bioreactor system to mimic the wall motion of the native heart muscle. A mouse ischemic injury model to test the ability of the AFS cells to survive and integrate after injection into the myocardium. AFS cell integration and function was evaluated by histology, echocardiography, and MRI. By 10 days, AFS cells stained positively for cardiomyocyte markers including troponin I, troponin T, α‐actinin. PCR analysis revealed expression of cardiomyocyte markers GATA‐4 and MEF2C. AFS‐derived cardiomyocytes preconditioned in the bioreactor showed muscle tissue organization and contraction. AFS cells injected into injured mouse hearts survived and integrated into the myocardium. Data showed a trend in functional improvement in the AFS cell treated group compared to ligated group without cells. These results demonstrate that multipotent stem cells derived from amniotic fluid may aid cardiac regeneration. AFS cells represent a promising novel source of cells for therapy of a variety of cardiac diseases.