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Gene expression analysis during pre‐implantation mouse embryo development using cDNA microarray
Author(s) -
Kim Hyun Jae,
Lee Seung Ku,
Cha YunYi,
Kim Hyoun Geun,
Yun HongShik,
Kang HyunJun,
Kim Kyoung Yeon,
Lee SookHwan,
Kwack KyuBum
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a224-c
Subject(s) - embryo , biology , microarray analysis techniques , gene , gene expression , microarray , complementary dna , reprogramming , embryonic stem cell , embryogenesis , gene expression profiling , transcriptome , microbiology and biotechnology , dna microarray , genetics
To improve our understanding of the molecular mechanisms underlying early embryo development, further characterization of gene activity in oocytes and embryos is urgently required. The transition from the two‐cell to four‐cell stage is particularly important in pre‐implantation embryonic development, as it involves transcriptional reprogramming and cellular differentiation. In this study, we used a 7.4 K cDNA microarray to screen mRNA transcript levels in the pre‐implantation mouse embryo. Real‐time PCR was used to confirm microarray data. We profiled 7410 genes and identified 4562 genes that were differentially expressed in the pre‐implantation embryo. We selected a total of 248 genes with significant expression changes that are functionally involved in the two‐cell and two‐cell block embryo. Of these genes, 114 were down‐regulated and the remainder (n = 134) were up‐regulated in the two‐cell embryo. This study provides a developmental map of a large number of genes in the pre‐implantation mouse embryo with particular emphasis on gene expression in the two‐cell embryo and two‐cell block embryo. Further investigations based on this data will provide a better understanding of the effects of various external conditions and may facilitate comparative analysis of preimplantation development in other mammalian species, including human.