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Myogenic factors that regulate expression of myogenic microRNAs
Author(s) -
Rao Prakash K,
Kumar Roshan M,
Farkhondeh Mina,
Baskerville Scott,
Lodish Harvey
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a202-a
Subject(s) - myod , c2c12 , microrna , myogenin , myocyte , skeletal muscle , biology , microbiology and biotechnology , myogenesis , gene silencing , gene expression , gene , genetics , anatomy
MicroRNAs are small non‐coding RNAs that are important regulators of gene expression. MicroRNA profiling studies have determined a number of tissue‐specific microRNAs. miR‐1 and miR‐133 are examples of tissue‐specific microRNAs and are exclusively muscle‐specific being expressed only in the heart and skeletal muscle. miR‐206 is preferentially expressed in the skeletal muscle. We have determined the expression pattern of these tissue‐specific microRNAs in C2C12 cells in an effort to see whether we can use them to study their regulation in an in vitro cell culture system. We describe the induction of miR‐1 and miR‐133 expression specifically during the myogenic conversion of C2C12 myoblasts and in primary human myoblasts. Furthermore, we have used genomic location analyses to determine the occupancy of the myogenic factor MyoD and Myogenin in upstream regions of these microRNAs. Our data suggests that these myogenic factors are important in regulating the expression of miR‐1 and miR‐133 in C2C12 cells, and by extension, in normal skeletal muscle.