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Dimerization of Junctional Adhesion Molecule‐A (JAM‐A) regulates cell migration through prevention of β1 integrin proteosomal degradation.
Author(s) -
Severson Eric,
Jiang Liangyong,
Ivanov Andrei,
Mandell Kenneth,
Nusrat Asma,
Parkos Charles A
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a192-a
Subject(s) - integrin , microbiology and biotechnology , mg132 , lactacystin , fibronectin , cell migration , chemistry , proteasome inhibitor , integrin, beta 6 , cell adhesion , hek 293 cells , focal adhesion , cell culture , collagen receptor , cell , proteasome , extracellular matrix , biology , biochemistry , receptor , genetics
Junctional adhesion molecule‐A (JAM‐A) is a tight junction protein that has been implicated in the regulation of migration and spreading of epithelial cells, however, mechanistic studies are lacking. We investigated the effects of dimerization‐defective JAM‐A mutants that either lack the distal extracellular Ig loop (DL1) or the dimerization motif (6163) on 293T cell migration and spreading. While such cells express a small amount of wild‐type JAM‐A, overexpression of dimerization‐defective JAM‐A mutants inhibited cell migration toward fibronectin and decreased spreading. These changes were accompanied by decreased cellular levels of β1 integrin expression and decreased density of focal adhesions as determined by immunofluorescence. A role for β1 integrin in these JAM‐A mediated effects was supported in experiments demonstrating decreased cell migration in 293T cells treated with siRNA specific to β1 integrin and rescue of cell migration by overexpression of β1 integrin in the dimerization‐defective cell lines. β1 integrin mRNA levels in all of the cell lines were the same; however, further experiments determined that treatment of JAM‐A dimerization‐defective cells with proteosome inhibitors MG132 and lactacystin resulted in restoration of β1 integrin protein levels to that of controls. Taken together, these results indicate that dimerization of JAM‐A regulates cell migration and spreading by controlling β1 integrin protein stability through prevention of β1 integrin degradation by the proteosome. Supported by NIH R01 DK61379