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Protein expression profiling in the spectrum of renal tumors
Author(s) -
Valera Vladimir Alexander,
Tapia Elsa LiNing,
Teller Linda,
Roberts David D.,
Linehan W. Marston,
Merino Maria J.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a181-a
Subject(s) - renal cell carcinoma , microdissection , laser capture microdissection , biology , pathology , proteomics , microbiology and biotechnology , kidney , computational biology , medicine , gene expression , genetics , gene
Background: Several genetic and epigenetic alterations have been described in renal cell carcinomas (RCC) related to tumor initiation and progression. However, particular changes in the protein expression profiles of these tumors have not been described yet. Accurate knowledge of the repertoire of proteins associated with renal cancers will not only provide insights into these mechanisms but will provide new markers for early detection. Methods: After microdissection, enriched cell populations from frozen tissue sections derived from a spectrum of inherited and sporadic RCC patients, and a set of benign tumors and normal kidney were analyzed by 2‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE). Gels were digitally scanned, compared, and significant, differentially expressed spots were excised and analyzed by MALDI‐TOF MS. Results: Several protein spots were found statistically (p<0.05) up or down regulated between cancer and normal tissues. From 66 differentially expressed protein spots, 8 different polypeptides were successfully identified. Classification algorithms efficiently and reproducibly classified tumors by the spot pattern. Conclusions: Proteomic profiling by 2‐D PAGE identified a series of differentially expressed proteins between RCC and normal tissue. The analysis also highlights that the protein profile may eventually prove to be useful in the classification of renal tumors.