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Application in healthy subjects of an iron‐induced fecal oxidative response test, using an in vitro reactive oxygen species (ROS) generation system
Author(s) -
Orozco Monica N.,
Solomons Noel W.,
Friel James K.,
Schuemann Klaus
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a173
Subject(s) - chemistry , feces , ferrous , context (archaeology) , reactive oxygen species , palm oil , food science , zoology , biochemistry , biology , microbiology and biotechnology , paleontology , organic chemistry
The up to 60% of non‐absorbed dietary iron can participate in the generation of ROS in the colon. Measuring ROS production in fecal matter can be difficult and tedious. Our purpose was to measure the effect of supplemental iron on ROS production in human feces. Four healthy male subjects received 120 mg of iron as ferrous sulfate during 2 periods of 7 consecutive days; in the second period, 5 ml of refined palm oil were added to the iron dose. Stool samples were collected at baseline, during supplementation days, and 11 days later. They were analyzed using a calibrated HPLC method proposed by Owen, et al, 2000 , validated by a hypoxanthine/xanthine oxidase model. ROS production (expressed as mg of hydroxylated products resulting from the free‐radical attack on salicylic acid) showed a mean increase from 0.26 mg at baseline to 0.33 mg during supplementation and decreased to 0.27 mg after iron supplementation alone. With 5 ml of palm oil added to the iron dose, the response increased on average, from 0.27 mg at baseline to 0.35 mg during supplementation and decreased to 0.27 mg after supplementation. We concluded that the in vitro assay can be used in a human metabolic study context. Funded by MPOB