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The Trx2 transgene ameliorates age‐related oxidation of plasma Cys/CySS redox state in aging mice
Author(s) -
Craige Siobhan E,
Hansen Jason M,
Go YoungMi,
Jones Dean P
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a172-b
Subject(s) - chemistry , redox , cystine , cysteine , oxidative stress , glutathione , antioxidant , biochemistry , enzyme , inorganic chemistry
Oxidative stress is implicated in aging and age‐related disease. The redox state of the major plasma thiol/disulfide pool, cysteine (Cys) and its disulfide cystine (CySS), can be used as a marker of oxidative stress. An oxidation of the Cys/CySS redox state is correlated with aging and regulates cell proliferation apoptosis and differentiation. Furthermore, in aging humans, oxidation of the Cys/CySS redox state can be prevented by antioxidant supplementation. The purpose of the present study was to determine whether the plasma Cys/CySS redox state was oxidized in association with aging in mice and whether expression of the human antioxidant protein, thioredoxin2 (Trx2), would ameliorate Cys/CySS redox state oxidation. Cys and CySS concentrations were measured by HPLC analysis and Cys/CySS redox state was calculated by the Nernst equation (Eh (mV)=Eo + RT/nF(ln[Cyss]/[Cys]2). Results show that the plasma Cys/Cyss redox state was oxidized in aging mice (>20mos; 90.7 ± 1.9 mV) compared to young mice (<6mos; 95.2 ± 1.3 mV), and expression of the Trx2 transgene prevented oxidation of the Cys/CySS redox state.