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Flaxseed Lignans Protect Against Acid Aspiration‐Induced Acute Lung Injury In Vivo and H 2 O 2 ‐Induced Cell Death In Vitro
Author(s) -
Lee James C,
Arguiri Evguenia,
Solomides C. C.,
Solomidou Melpo Christofidou
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a172-a
Subject(s) - malondialdehyde , lipid peroxidation , oxidative stress , apoptosis , lignan , pharmacology , chemistry , in vivo , antioxidant , inflammation , biochemistry , medicine , immunology , biology , microbiology and biotechnology , stereochemistry
Aspiration of gastric contents is a common cause of respiratory distress. We have shown that dietary flaxseed (FS) protects from acid aspiration (AA)‐induced acute lung injury (ALI) in mice. Lignans are bioactive compounds in FS with known antioxidant properties and have not been evaluated in oxidative lung disease. We tested dietary Flaxseed Lignan Complex (FLC), containing the lignan precursor secoisolarisilesinol diglycoside (SDG), in a murine model of AA‐induced ALI. Mice were pre‐fed either FLC‐supplemented or isocaloric control‐diets. Lungs were evaluated 24 hours post intra‐tracheal 0.1N HCl instillation for AA‐induced injury, inflammation, and lipid peroxidation by measuring malondialdehyde (MDA) levels. FLC‐fed mice showed trends towards decreased lung edema and inflammation as measured by BAL protein content, and WBC and % neutrophils counts. MDA was significantly decreased in the FLC fed mice compared to control diet fed mice after AA (8.3 vs. 3.8 μM/g protein). Additionally, both cultured alveolar epithelial cells and isolated murine primary pulmonary microvascular endothelial cells preincubated with purified mammalian FS lignans prior to H 2 O 2 challenge were protected in a dose dependent manner from cell death as measured by lactate dehydrogenase release. We conclude that dietary FS lignan precursor reduces oxidative lung damage and purified mammalian FS lignans ameliorate oxidative cell death. FS lignans may be safe, adjuvant therapeutic agents in oxidative lung disease. Funded by: NIHR21, AICR and U. Penn Research Foundation (MCS).