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Cultured cells differ in their homeostatic response to zinc deprivation
Author(s) -
Dutta Amrita,
Grattan Bruce,
Sankavaram Kavitha,
Freake Hedley C
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a170-b
Subject(s) - zinc , efflux , cell culture , extracellular , chemistry , homeostasis , cell , anterior pituitary , endocrinology , medicine , pituitary gland , biochemistry , biology , hormone , organic chemistry , genetics
Previous studies from our laboratory with radioactive Zn‐65 have shown that H4IIE (rat hepatoma) and GH3 (rat pituitary tumor) cell lines reduce zinc efflux in the presence of the extracellular metal chelator DTPA (diethylenetriaminepentaacetic acid) whereas primary rat hepatocytes give out more zinc under the same conditions. To further understand this difference, the effects of DTPA on radioactive Zn‐65 flux were examined in primary rat anterior pituitary cells and both MCF‐7 (human breast cancer) and Hs578Bst (human normal mammary) cell lines. When cells were co‐incubated with DTPA (50 uM) and Zn‐65, the chelator reduced uptake of label in all cells examined. When primary pituitary cells were pre‐labeled with Zn‐65 and then incubated with DTPA, the chelator increased efflux of Zn‐65. Thus the rat anterior pituitary cells behaved similarly to the primary hepatocytes and differently from the GH3 cells. However, DTPA resulted in increased retention of Zn‐65 in both pre‐labeled MCF‐7 and Hs578Bst cells. Thus, the cellular responses to zinc deprivation differ between the established cell lines, which increase zinc retention, and the primary cells, which give out more zinc. This may be related to the enhanced zinc requirements of rapidly growing cells.