Premium
Discovery of two eukaryotic nicotinamide riboside salvage pathways: New nutritional approaches to promote Sir2 functions
Author(s) -
Brenner Charles
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a158-d
Subject(s) - nad+ kinase , riboside , nicotinamide , nicotinamide mononucleotide , nicotinamide adenine dinucleotide , biochemistry , yeast , niacinamide , saccharomyces cerevisiae , nucleotide salvage , biology , chemistry , nicotinamide phosphoribosyltransferase , gene silencing , gene , microbiology and biotechnology , enzyme , nucleotide
We recently discovered that nicotinamide riboside, previously thought to be a bacterial NAD precursor, is a vitamin precursor of NAD in yeast and humans, which is converted to NAD in a pathway initiated by specific nicotinamide riboside kinases. Nicotinamide riboside has been shown to allow dorsal ganglion root neurons to resist axonopathy and the nicotinamide riboside kinase 2 gene has been shown to be induced by damage. In this study, we describe a second biosynthetic pathway for nicotinamide riboside salvage in yeast and human cells. We establish the conditions in which yeast cells take up the compound and convert it to NAD and we show that both of the newly identified nicotinamide riboside salvage pathways increase Sir2 activity in gene silencing and promote yeast cell longevity in the absence of calorie restriction.