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Fluorescence imaging of cell signaling systems
Author(s) -
Meyer Tobias
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a150-a
Subject(s) - förster resonance energy transfer , second messenger system , signal transduction , endoplasmic reticulum , live cell imaging , microbiology and biotechnology , cell , cell signaling , fluorescence lifetime imaging microscopy , fluorescence , chemistry , biophysics , biology , biochemistry , physics , quantum mechanics
Quantitative fluorescence imaging provides powerful measurement opportunities for understanding cellular signaling pathways and networks. I will discuss in my talk different currently useful fluorescence biosensors and imaging strategies and will present some of our recent results in applying fixed cell and live cell imaging to investigate the spatial and temporal control of second messenger signaling systems. My talk will have two parts, I will focus on a strategy that we developed to rapidly perturb phosphoinositide signaling pathways using chemically‐induced enzyme activation that led to new insights into the roles of PI(45)P2 and PI(345)P3 lipid second messengers in cell migration and chemotaxis. Second, I will discuss fluorescence‐imaging based siRNA screens that led to the discovery of STIM1 that sense Ca2+ in the endoplasmic reticulum and I will present recent work on using fluorescence energy resonance transfer to understand STIM1 signal transduction from the lumen of the ER to ER‐PM junctions.