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A Structure for Little Orphan Diacylglycerol Kinase
Author(s) -
Sanders Charles R,
Sonnichsen Frank D,
Kim Hak Jun
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a148-a
Subject(s) - diacylglycerol kinase , transmembrane protein , chemistry , micelle , enzyme , kinase , biochemistry , protein kinase c , organic chemistry , receptor , aqueous solution
The solution NMR‐determined structure of E. coli diacylglycerol kinase (DAGK) is described. DAGK is a 40 kDa homotrimeric enzyme that contains 9 transmembrane helices. NMR studies were conducted under conditions in which DAGK is a ca. 100 kDa complex with dodecylphosphocholine micelles. The technical hurdles that were encountered and overcome in this decade‐long project are described. This work was supported by the US NIH (RO1 GM47485). We thank James Bowie for providing the expression systems that led to this work, Kirill Oxenoid for his work in optimizing sample conditions and assigning DAGK's NMR spectrum, and Lewis Kay for advice on NMR.