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The effect of dose size on bioavailability of acylated and nonacylated anthocyanins from red cabbage
Author(s) -
Charron Craig S.,
Clevidence Beverly A.,
Britz Steven J.,
Novotny Janet A.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a120-b
Subject(s) - bioavailability , brassica , urine , excretion , biology , chemistry , biochemistry , pharmacology , botany
Recent studies indicate that dietary anthocyanins (ACN) provide health benefits, which depend on absorption and metabolic mechanisms that deliver ACNs and their bioactive metabolites to responsive tissues. We evaluated red cabbage (Brassica oleracea L. var. capitata) ACN bioavailability as reflected by urinary excretion of ACNs and ACN metabolites. Twelve volunteers consumed 100 g, 200 g, and 300 g of red cabbage (containing 1.09 μmol of ACNs/g cabbage) in a crossover design. ACN concentration in cabbage extract and urine was measured by HPLC‐MS/MS. Six nonacylated and 30 acylated ACNs were detected in red cabbage, and 2 nonacylated ACNs, 8 acylated ACNs, and 5 metabolites were present in urine. Mean 24‐h excretion of intact ACNs increased linearly from 45 (100‐g dose) to 65 μmol (300‐g dose) for acylated ACNs and from 52 (100‐g dose) to 79 μmol (300‐g dose) for nonacylated ACNs. Urinary recovery of intact ACNs (% of ACN intake) decreased linearly from 0.041% (100‐g dose) to 0.019% (300‐g dose) for acylated ACNs and from 0.176% (100‐g dose) to 0.085% (300‐g dose) for nonacylated ACNs. ACN metabolites consisted of glucuronidated and methylated ACNs. Our results show that red cabbage ACNs were excreted in both intact and metabolized forms and that recovery of nonacylated ACNs in urine was over 4‐fold that of acylated ACNs. This work was supported by the U.S. Department of Agriculture.