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In utero and postpartum exposure to long chain n‐3 polyunsaturated fatty acids improves intestinal glucose absorption and glycogen stores in weanling pig
Author(s) -
Gabler Nicholas K,
Spencer Joel D,
Webel Doug M,
Spurlock Michael E
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a119
Subject(s) - weanling , glut2 , jejunum , glycogen , polyunsaturated fatty acid , docosahexaenoic acid , in utero , fish oil , medicine , endocrinology , glucose transporter , fatty acid , glucose uptake , chemistry , zoology , biology , biochemistry , pregnancy , fetus , insulin , fishery , fish <actinopterygii> , genetics
The objective of this study was to determine whether in utero and postnatal exposure of long chain n3‐PUFA would alter glucose absorption from the proximal jejunum or muscle (longissimus dorsi) glycogen concentrations in piglets at weaning. Dams were fed one of three experimental diets for 150 days prior to farrowing: 1) corn/soybean meal control; 2) As 1 + protected fish oil, (PFO, JBS United Inc.) or, 3) As 1 + algal cells high in docosahexaenoic acid (DHA, ABN Corp.). Treatments 2 and 3 were formulated to the same level of DHA. All tissues were collected from piglets weaned and fasted for 24 h (n=4/trt). Jejunum and muscle samples were analysed for fatty acid profiles which reflected the dam's dietary and milk fatty acid profile. Proximal jejunum samples were mounted in modified Ussing chambers. Piglets from treatments 2 and 3 displayed increased (P<.05) active glucose transport (133 and 420%, respectively) compared to treatment 1. The improvement in glucose uptake associated with trts 2 and 3 coincided with increased (P<.05) GLUT2 and SGLT1 protein expression. However, muscle glycogen was increased (P<.05) only in piglets from dams allotted to trt 3 (46 vs 34 mg/g wet tissue, for trts 1 and 3, respectively). These results indicate that the absorption and storage of glucose may be enhanced by in utero and neonatal exposure to long chain n3‐PUFA such as DHA.