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Decosahexaenoic Acid (DHA) but not Eicosapentaenoic Acid (EPA) Reverses trans‐10, cis‐12‐Conjugated Linoleic Acid (t10, c12‐CLA) Induced Insulin Resistance in Mice
Author(s) -
Vemuri Madhuri,
Kelley Darshan S,
Bartolini Giovanni,
Rasooly Reuven,
Mackey Bruce
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a113-a
Subject(s) - eicosapentaenoic acid , insulin resistance , conjugated linoleic acid , docosahexaenoic acid , fish oil , fatty acid , chemistry , linoleic acid , polyunsaturated fatty acid , insulin , medicine , food science , biology , biochemistry , endocrinology , fish <actinopterygii> , fishery
Fish oil added to CLA containing diets has been shown to reduce fatty liver. DHA and EPA are the primary fatty acids in fish oil. We conducted this study to determine if DHA, EPA or both can reverse fatty liver and insulin resistance caused by t10, c12‐CLA. Eight week old, female mice (n=8–10/group) were fed either a control diet, or diets supplemented with 0.5% t10, c12‐CLA alone or in combination with 1.5% DHA or 1.5% EPA for eight weeks. There was no significant difference in food intake, body weight or plasma glucose between the groups. Compared to the control group, there was a 270% and 172% increase in the liver weights of animals fed t10, c12‐CLA and t10, c12‐CLA + EPA, respectively; but liver weights in t10, c12‐CLA + DHA group did not differ significantly from control group. Insulin resistance as measured by HOMA‐IR values were significantly (p<0.0001) higher in t10, c12‐CLA (6.73±2.73, Mean±SEM) and t10, c12‐CLA + EPA (9.096±1.93) groups than control group (0.46±0.11). But HOMA‐IR in t10, c12‐CLA + DHA group (1.92±0.24) was not significantly different than that in control group. Thus, the data suggest that DHA and not EPA in fish oils reverse t10, c12‐CLA induced fatty liver and insulin resistance.