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The Impact of Different Ratios of Omega‐6 Polyunsaturated Fatty Acids to Eicosapentaenoic acid (EPA) plus Docosahexaenoic acid (DHA) on Atherosclerotic Lesion Formation and Inflammatory Factors in the LDL receptor knockout (LDLr−/ −) mouse
Author(s) -
Wang Shu,
Wu Dayong,
Matthan Nirupa,
Lichtenstein Alice
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a108-a
Subject(s) - eicosapentaenoic acid , docosahexaenoic acid , medicine , chemistry , endocrinology , polyunsaturated fatty acid , ldl receptor , cholesterol , fatty acid , lipoprotein , biochemistry
The objective was to assess the effect of different ratios of omega‐6 to EPA plus DHA on atherosclerotic lesion formation and plasma inflammatory markers in LDLr−/− mice. Mice (n=10/group) were fed the following diets for 32 weeks: high fat (20% w/w) without EPA and DHA (HF omega‐6), and high fat with omega‐6 to EPA plus DHA ratios of 20:1 (HF R20:1), 4:1 (HF R4:1), and 1:1 (HF R1:1). Serum total cholesterol (mg/dL) was significantly (P<0.05) lower in the mice fed HF R4:1 (857 ± 100) and HF R1:1 (646 ± 109) compared to the mice fed HF omega‐6 (1069 ± 107) and HF R20:1 (1109 ± 184). Serum non‐HDL cholesterol levels showed the same trend. Aortic total cholesterol was 2.6%, 19.8% and 24.3% lower in the HF R20:1, HF R4:1 and HF R1:1 fed mice, respectively, compared with HF omega‐6 fed mice. Plasma monocyte chemoattractant protein‐1 was 88.3 ± 56.7, 86.3 ± 58.6, 73.1 ± 25.0 and 63.6 ± 28.1 pg/mL, and plasma interleukin‐6 was 41.8 ±15.4 a , 30.9 ± 15.7 ab , 26.9 ± 15.2 b , and 23.6 ± 8.9 b pg/mL (values with different superscripts are significantly different at P<0.05) in mice fed HF omega‐6, HF R20:1, HF R4:1, and HF R1:1 diets, respectively. These data suggest that diets with lower omega‐6 to EPA plus DHA ratios resulted in lower levels of serum total and non‐HDL cholesterol, and plasma inflammatory markers which in turn were associated with less aortic lesion formation. The research was supported by U.S. Department of Agriculture, No. 58‐1950‐4‐401.