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Vitamin C Transporter Gene SLC23A1 Polymorphisms and Fasting Plasma Ascorbate
Author(s) -
Cahill Leah,
FontaineBisson Bénédicte,
ElSohemy Ahmed
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a105
Subject(s) - genotype , medicine , endocrinology , allele , vitamin c , ascorbic acid , polymorphism (computer science) , vitamin , biology , gene , chemistry , genetics , food science
Vitamin C transporter protein SVCT1, encoded by the SLC23A1 gene, is required for intestinal absorption and renal reabsorption of vitamin C in humans. The purpose of this study was to determine whether polymorphisms of SLC23A1 alter fasting plasma concentrations of ascorbate (vitamin C). We recruited 406 non‐smokers aged 20–29 years. Blood samples were collected to determine fasting plasma ascorbate concentrations and to isolate DNA to determine SLC23A1 genotypes (1091T>C and 2515G>C) by real‐time PCR. For the 1091T>C polymorphism, the frequency of the C allele differed between Caucasians (63%), Asians (29%) and South East Asians (49%) (p<0.0001). The frequency of the C allele for the 2515G>C polymorphism also differed between Caucasians (34%), Asians (64%) and South East Asians (49%) (p<0.0001). However, average plasma ascorbate concentrations (mean ± SD μmol/L) did not vary between the TT (30.2±13.4), TC (32.8±14.8) and CC (29.8±14.6) genotypes for the 1091T>C polymorphism (p = 0.22), or between the GG (30.8±15.9), GC (31.8±13.7) and CC (30.8±13.8) genotypes for the 2515G>C polymorphism (p = 0.52). These associations were not modified by sex or ethnicity. Our findings indicate that two common polymorphisms of the SLC23A1 gene do not affect concentrations of fasting plasma ascorbate. The role of SLC23A1 genotypes in modifying the association between dietary and plasma ascorbate remains to be determined. (Supported by AFMNet.)