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Elucidating the role of adenine nucleotide transporter in mitochondrial swelling: an experimental and computational approaches
Author(s) -
ChapaDubocq Xavier R.,
GarciaBaez Jorge F.,
Bazil Jason N.,
Javadov Sabzali R.
Publication year - 2022
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2022.36.s1.r5305
Subject(s) - mitochondrion , adenine nucleotide translocator , bioenergetics , mptp , oxidative phosphorylation , mitochondrial permeability transition pore , voltage dependent anion channel , microbiology and biotechnology , ant , inner mitochondrial membrane , chemistry , adenine nucleotide , biophysics , mitochondrial membrane transport protein , respiratory chain , mitochondrial matrix , membrane potential , biochemistry , cytosol , programmed cell death , biology , bacterial outer membrane , nucleotide , apoptosis , enzyme , neuroscience , ecology , dopaminergic , escherichia coli , gene , dopamine
Mitochondria produce over 90% of cellular ATP and actively participate in maintaining ion homeostasis, redox status, lipid metabolism, and cell growth. Changes in the matrix volume of mitochondria affect their functional and structural integrity. Modest volume increases associated with modulation of the inner mitochondrial membrane can activate electron transfer chain and oxidative phosphorylation, whereas excessive swelling impairs structural organization of the membrane and initiate mitochondria‐mediated cell death mechanisms. Therefore, clarifying the precise mechanisms of excessive mitochondrial swelling is important for regulation of mitochondria‐mediated cell death pathways in response to energy and oxidative stresses. Opening of non‐selective mitochondrial permeability transition pores (mPTP) is the primary cause of excessive matrix swelling. The molecular identity remains unknown and recent studies suggest the existence of two or more types of mPTP that can be composed of different protein(s). The adenine nucleotide translocator (ANT) and F O F 1 ‐ATP synthase were proposed to be potential mPTP core components that can act together or independently each other. Here, we elucidated the role of ANT in mPTP opening by applying both experimental and computational approaches. mPTP opening was evaluated in cardiac mitochondria that were exposed to moderate and high Ca 2+ concentrations in the absence and presence of respiratory substrates and ADP. We developed a detailed model of the ANT transport mechanism including the matrix (ANT M ), cytosolic (ANT C ), and pore (ANT P ) states of the transporter that was able to simulate our experimental data. In addition, we corroborated and simulated our ANT model based on previous ANT kinetics data. The model was successful not only in simulating ANT pore state transition, but also explained the potential role of ANT in mPTP opening in cardiac mitochondria.