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Neucode Tags for Highly Multiplexed Metabolomics
Author(s) -
Grady Scott,
Armbruster Michael,
Edwards James,
Arnatt Christopher
Publication year - 2022
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2022.36.s1.r4892
Subject(s) - metabolomics , chemistry , linker , mass spectrometry , amine gas treating , multiplexing , aldehyde , combinatorial chemistry , computational biology , chromatography , biochemistry , biology , computer science , organic chemistry , operating system , telecommunications , catalysis
We describe the design and synthesis for neutron encoded (NeuCode) tags for highly multiplexed targeted sample analysis for use in high resolution mass spectrometry (HRMS) based metabolomics. A set of aldehyde and carboxylic acid reactive tags were synthesized with a quaternary ammonium polar head group which limits off‐target ionization and promotes coelution of differentially tagged metabolites. A four carbon linker allowed us to synthesize highly multiplexed NeuCode tags by providing many areas for isotope substitution and the obtained amine tags can be easily converted to other functional groups to target a wide array of metabolites. Tags were used to analyze levels of targeted metabolites in differentially treated cell lysates producing tagged samples which were mixed 1:1 and run in a single LC‐MS injection.