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F1A‐CreER T2 Mice: Targeting and Tracing Cardiomyocytes with Fgf1 Expression in Adult Mouse
Author(s) -
Chiu IngMing,
Chung YuFen,
Chen MeiShu,
Wang ChiKuang,
Jiang SiTse,
Hsu YiChao
Publication year - 2022
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2022.36.s1.r4302
Subject(s) - exon , microbiology and biotechnology , biology , messenger rna , in vivo , untranslated region , indel , genetically modified mouse , fgf1 , gene , chemistry , fibroblast growth factor , transgene , biochemistry , genetics , receptor , fibroblast growth factor receptor , genotype , single nucleotide polymorphism
Fibroblast growth factor 1 (FGF1) regulates many biological and homeostatic processes, including obesity and metabolic syndrome. In mice, Fgf1 gene contains at least three upstream promoters and their respective adjacent untranslated exons that spliced to the first protein coding exon mutually exclusively, giving rise to different Fgf1 mRNA variants. Among them, the Fgf1A transcript is predominantly expressed in the heart. FGF1 can induce cardiomyocyte regeneration and cardiogenesis in vitro and in vivo . Here, we generated a novel mouse line Fgf1A promoter (F1A)‐driven inducible Cre recombinase (CreER T2 ). We firstly demonstrated that the highest mRNA expression of CreER T2 were detected in the heart specifically of F1A‐CreER T2 mice, similar to that of Fgf1A mRNA. The F1A‐CreER T2 mice were crossed with ROSA26 mice, and the F1 mice were analyzed. The LacZ‐positive signals were detected exclusively in the heart after tamoxifen administration. The CreER T2 ‐mediated recombination in the tissues is monitored through LacZ‐positive signals, indicating the in situ localization of F1A‐positive cells. Consistently, these F1A‐positive cells with RFP‐positive signals or LacZ‐positive blue signals were co‐localized with cardiomyocytes expressing cardiac troponin T, suggesting cardiomyocyte‐specific activation of Fgf1A promoter. Our data suggested that the F1A‐CreER T2 mouse line could be used for time‐dependent and lineage tracing of Fgf1A‐expressing cells in vivo . F1A‐CreERT2 line is a valuable tool for in vivo lineage tracing of Fgf1A‐expressing cells during heart development and will facilitate the elucidation of the physiological and pathological role of Fgf1A in heart failure and metabolic syndrome.