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The Effects of Prune Extract on Cellular Models of Bone Cancer
Author(s) -
Miller Chelsie,
Adam Mowaffaq,
Chao Grace,
Hooshmand Shirin,
Sohl Christal
Publication year - 2022
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2022.36.s1.r2210
Subject(s) - antioxidant , polyphenol , cell , chemistry , cell growth , cell culture , bone health , cancer research , microbiology and biotechnology , biology , biochemistry , medicine , pathology , bone mineral , genetics , osteoporosis
There is strong evidence for prunes as a health food for improvement in digestion due to their high fiber content. More recent work has shown that prunes also have a positive impact on bone health, attributed in part to the antioxidant compounds and polyphenols they possess. Despite their important antioxidant role, the anticancer properties of prunes has yet to be determined. We hypothesize that treatment of osteosarcoma cell lines with prune extracts will lead to a decrease in cell proliferation and migration, driven at least in part due to the antioxidant compounds. We will treat C28, Saos‐2, and hFOB 1.19 bone cell lines with varying concentrations of prune extract, and use the CellTiterGlo assay and scratch assays to measure the effects on cell proliferation and migration, respectively. A key component of our work is establishing the optimum cell culturing conditions and developing robust protocols for important cancer phenotype assays. Upon completion of these experiments, we hope to better understand the effects of prunes on bone health and disease.