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Purification of the human cone‐rod homeobox protein
Author(s) -
Hayes Jonathan E.,
Enke Raymond A.,
Berndsen Christopher
Publication year - 2022
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2022.36.s1.l7459
Subject(s) - transcription factor , chemistry , homeobox , mutant , protein domain , microbiology and biotechnology , gene , biophysics , biology , biochemistry
A “lock and key” is the most common depiction of protein binding, protein has a specific structure, and its binding molecule of interest can fit into it. What happens when a protein domain needs to bind to multiple biomolecules? The transcription factor CRX is one of those with an activation domain that can bind to various biomolecules. CRX regulates the gene expression of cone and rod photoreceptors. To effectively regulate gene expression, CRX’s activation domain interacts with multiple transcriptional co‐activators. Unlike the “lock and key” concept with defined structures, we propose that the activation domain of CRX is intrinsically disordered, allowing for interactions of multiple proteins in the same domain. In support of a disordered activation domain, we observe that proline and serine amino acids are abundant in the CRX activation domain. We also note an abundance of pathogenic frameshift mutations and a lack of point mutants in the activation domain. To characterize the structure of CRX, we attempted to purify full‐length CRX and CRX coexpressed with known transcriptional coactivator NRL. We hypothesize that this latter experiment will enhance the stability and expression of full‐length CRX. We have purified CRX using nickel chromatography and are currently optimizing the next steps to enhance the purity of CRX and NRL. The information from biochemical and structural experiments on purified CRX will provide insight into the transcriptional regulation of CRX, leading to the development of photoreceptor neurons.