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MED1 is a lipogenesis coactivator required for postnatal adipose expansion
Author(s) -
Jang Younghoon
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.04782
Subject(s) - coactivator , mediator , lipogenesis , adipogenesis , adipose tissue , enhancer , biology , transcription factor , endocrinology , medicine , microbiology and biotechnology , gene , genetics
MED1 often serves as a surrogate of the general transcription coactivator complex Mediator for identifying active enhancers. MED1 is required for phenotypic conversion of fibroblasts to adipocytes in vitro but its role in adipose development and expansion in vivo has not been reported. Here we show that MED1 is dispensable for adipose development in mice. Instead, MED1 is required for postnatal adipose expansion and the induction of de novo lipogenesis (DNL) genes after pups switch diet from high‐fat maternal milk to carbohydrate‐based chow. During adipogenesis, MED1 is dispensable for induction of lineage‐determining transcription factors (TFs) PPARγ and C/EBPα but is required for lipid accumulation in the late phase of differentiation. Mechanistically, MED1 controls the induction of DNL genes by facilitating lipogenic TF ChREBP‐dependent recruitment of Mediator to active enhancers. Together, our ndings identify a cell‐ and gene‐specific regulatory role of MED1 as a lipogenesis coactivator required for postnatal adipose expansion.