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Temperature Dependence on the Passive Effects of K+ on Membrane Potential of Skeletal Muscle as an Educational Module
Author(s) -
Buendia Castillo Dara,
Krall Rebecca,
Chalfant Jeffrey,
Sharp Katherine,
Cooper Robin
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.04702
Subject(s) - membrane potential , membrane , chemistry , comprehension , protocol (science) , skeletal muscle , crayfish , nernst equation , representation (politics) , mathematics education , computer science , psychology , thermodynamics , biochemistry , physics , ecology , anatomy , biology , medicine , pathology , alternative medicine , electrode , politics , political science , law , programming language
The presentation reports on the implementation of a CURE (Course‐based Undergraduate Research Experience) by modifying a standardized physiological laboratory protocol that measures changes in resting membrane potential concerning the concentration of extracellular potassium ions [K + ] o . The CURE investigation determines how temperature influences resting membrane potential. Crayfish were used as models to empirically address the effects of temperature and [K + ] o change. Experimentally, the skeletal muscle of a crayfish served as a model to obtain data due to the robust nature of the preparation for student laboratories. Graphing membrane potential in relation to both [K + ] o and temperature, along with theoretical curves for the Nernst and Goldman‐Hodgkin‐Katz (G‐H‐K) equations, provided a visual representation of the significance of temperature on membrane potential and in these equations. Freely available online software was used in addressing the theoretical values one would expect for changing the variables in the G‐H‐K equation, which gave students a deeper understanding of its use in measuring membrane potential values. A pre‐ and post‐survey over content comprehension and student views on the activities were also obtained.