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Evolutionary Glycomics: A Comprehensive Study of Vertebrate Host Serum/Plasma Glycome Using Orthogonal Glycomics Techniques
Author(s) -
Murugan Abarna V. M.,
Oliveira Tiago,
Alagesan Kathirvel,
Mojtahedinyazdi Yasin,
Mariethoz Julien,
Hayes Catherine,
Lisacek Frédérique,
Karlsson Niclas,
Nothaft Harold,
Szymanski Christine,
Finlayson Kimberly,
Merwe Jason,
Richardson Samantha,
Kolarich Daniel
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.04538
Subject(s) - glycome , glycomics , glycan , vertebrate , fucosylation , glycosylation , biology , sialic acid , epitope , fucose , pngase f , host (biology) , chemistry , biochemistry , galactose , glycoprotein , genetics , antibody , gene
Eukaryotic Vertebrate host glycosylation machinery has been subject to immense evolutionary pressure. Besides, being crucial for host sustenance, glyco‐epitopes are also key‐regulators of inter‐species pathogen transmission. Significant evolutionary events such as loss of N‐glycolyl neuraminic acid (Neu5Gc) or alpha Galactose (α‐Gal) epitopes in species such as humans have been relevant for species development and have significantly impacted the ongoing arms race between pathogen and their hosts. Comprehensive knowledge on Vertebrate species‐specific glycosylation is still scattered, thereby leaving a substantial gap in our understanding of their glycome evolution. Serum/plasma N‐ and O‐glycomes of 35 different Vertebrate species (mammals, marsupials, birds, reptiles and fish) were analysed by two orthogonal glycomics methods, Porous Graphitised Carbon nano‐ Liquid Chromatography coupled to Electrospray Ionisation Tandem Mass Spectrometry (PGC nano‐LC ESI‐MS/MS) and Matrix Associated Laser Desorption Ionisation Time‐of‐flight Mass Spectrometry (MALDI TOF MS). Sialic acid diversity was additionally captured using High Performance Liquid Chromatography (HPLC) analyses of 1,2 Diamino Methylenedioxybenzene dichloride (DMB)‐ labelled sialic acids. Vertebrate serum/plasma glycome predominantly contained complex di‐, mono sialylated glycans. Core fucosylation of these N‐glycans was strongly species dependent, as were the non‐reducing end modifications. Most species showed a preference for incorporation of either N‐acetyl neuraminic acid (NeuAc) or NeuGc, with exceptions such as rats that use both. Anseriformes (Goose, Duck) N‐glycans carried GalNAc‐GlcNAc (LacDiNAc) epitopes that, however, were absent in Galliformes (Chicken, Turkey). Reptilians (e.g. Green sea turtles) had high levels of α‐Gal epitopes, and Saltwater Crocodile uniquely showed high amounts of oligomannose N‐glycans in serum. This largest, vertebrate species‐specific serum/plasma glycan and MS/MS spectral data is currently in the process of being made available open access through our collaboration with glycan focussed database such as Glyconnect and UniCarbDB.