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Single cell trajectories reveal a developmental sequence from angiogenic capillary progenitors to mature high endothelial cells
Author(s) -
Brulois Kevin,
Rajaraman Anusha,
Szade Agata,
Nordling Sofia,
Takeda Akira,
Jalkanen Sirpa,
Dermadi Denis,
Pan Junliang,
Butcher Eugene
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.04300
Subject(s) - biology , progenitor cell , immune system , neogenesis , angiogenesis , microbiology and biotechnology , transcriptome , stem cell , homeostasis , lymphangiogenesis , lymphatic system , immunology , progenitor , phenotype , gene , gene expression , genetics , endocrinology , islet , cancer , insulin , metastasis
Blood vascular endothelial cells (BECs) control the immune response by regulating blood ow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we prole transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets including a capillary resident progenitor (CRP) that displays stem cell and migratory gene signatures and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature HEC and arterial populations. Integrated analysis of mouse and human datasets reveals conserved cellular subsets and shared and divergent gene expression programs with implications for species differences in immune trafficking mechanisms. Our analyses provides a molecular roadmap of the lymph node blood vasculature and denes subset specialization for leukocyte recruitment and vascular homeostasis.