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Writing a review on trapping RNase substrate complexes for structural studies as an undergraduate researcher
Author(s) -
Jones Seth,
Gleghorn Michael,
Goossen Christian,
Lewis Sean,
Delaney Annie
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.03879
Subject(s) - rnase p , rnase h , ribonuclease , substrate (aquarium) , nucleic acid , cleavage (geology) , computational biology , rna , chemistry , computer science , nanotechnology , biology , biochemistry , materials science , ecology , paleontology , fracture (geology) , gene
We conducted a major survey of ribonuclease (RNase) substrate complex structures to identify strategies used to inhibit cleavage of the RNA. This review is intended to serve as a guide for structural biologists interested in discovering mechanisms and functions of ribonucleases through structural studies. We searched the Protein Data Bank for RNase structures which include both RNase and nucleic acid substrate and vetted the results for structures in which the substrate remained intact. Then we organized the structures by category based on the method used for inhibition. Each of the methods include their owns pros and cons and often it is advantageous to use a mix of different methods to achieve inhibition. In addition to surveying current strategies, we identify some ideas for future directions in the field based on what we have learned.