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Transcriptional Comparisons of Diverse Cancer Cell Types With Metastatic and Non‐Metastatic Pairings
Author(s) -
Kuehn Kirby,
Tauber Taylor,
Van Scoyk Christian,
Rech Tondin Arthur,
Broske Morgan,
Schreiner Cody,
Dresang Lindsay
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.03742
Subject(s) - metastasis , transcriptome , colorectal cancer , biology , merkel cell carcinoma , cancer research , cancer , cell type , merkel cell , pancreatic cancer , cancer cell , cell culture , cell , pathology , medicine , carcinoma , gene , gene expression , genetics
Hundreds of different cancer types exist because there are as many differentiated cells in the human body. Regardless of cancer type, liver metastasis often results in a poor prognosis given its myriad of essential functions. Six human cancer cell lines were grown: two each of Merkel cell, colorectal, and pancreatic carcinoma origin. Each pair was previously shown to either metastasize to the liver or not in a NOD‐SCID‐Gamma mouse xenotransplant model. RNA was isolated from these cells and a normal control (fibroblasts) for NovaSeq transcriptome analysis. Comparisons are available for transcripts differentially‐expressed from: 1) colorectal, 2) Merkel cell, and 3) pancreatic carcinomas, along with the normal control. Each cell line was assessed in triplicate. Nearly 4,000 transcripts were significantly up‐ or down‐regulated by 2‐fold or more in the cancer cells which metastasized to the liver in the mouse model, relative to their non‐metastatic counterparts. These transcripts were further assessed for pathways of significance in metastasis using gene ontology.