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Vascular Network Imaging on Whole‐Mount Mouse Dura Mater with Cranial Bone
Author(s) -
Xie Leike,
Sinha Sunilima,
Glinsky Vladislav,
Palaniappan Kannappan,
Glinskii Olga
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.03659
Subject(s) - magnification , skull , anatomy , dura mater , lymphatic system , medicine , pathology , sagittal suture , vascular network , computer science , computer vision
Intracranial vascular networks including both blood and lymphatic vessels are essential for the brain homeostasis and may change in structure or density under pathological conditions. Investigations on intracranial vascular networks require vasculature integrity in prepared samples. In the present study, we have established a method for acquiring vascular network images on a whole‐mount mouse dura mater with cranial bone. Intracardial perfusion on sacrificed six‐week‐old mice was carried out with fluorescently tagged soybean agglutinin (SBA) and anti‐LYVE‐1 antibody labeling blood and lymphatic vessel respectively. Following dissection and fixation, the dura mater with the skull was mounted on a cover glass. A series of individual images at 512 x 512 pixels were taken under a confocal microscope for subsequent generation of the whole‐mount mosaic image. For images at 20x magnification, 224 individual images were needed to cover the whole sample, including frontal and parietal areas, coronal suture, transverse and sagittal sinuses. For the 4x magnification, 12 images were required to cover the same sample area. Images at higher magnification revealed more clear and precise vascular network structural details. This method is useful in studying the relationship between the structural status of intracranial vascular networks and neurological disorders.