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Dose‐dependent Improvement in Cardiovascular Complications and Hyperglycemia Reversal Post‐mesenchymal Stem Cells Delivery in Diabetic Infarcted Rats
Author(s) -
Jabeen Sara,
Vishwakarma Sandeep,
Khan Aleem,
Gupta Usha
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.02669
Subject(s) - medicine , diabetes mellitus , streptozotocin , myocardial infarction , mesenchymal stem cell , insulin , endocrinology , pathology
Study objective To study the effect of intravenous delivery of single and double doses of mesenchymal stem cells (MSCs) on cardiovascular complications and hyperglycemia in diabetic infarcted rats. Hypothesis Diabetes has been one of the leading causes of deaths, and majority of these deaths are concomitant with myocardial infarction. Currently available clinical management strategies have failed to provide desired support to respective failing organs and reduce continuous increasing mortality rate. Hence, in this study improvement in cardiovascular complications and diabetic conditions were assessed in diabetic infarcted rats post‐MSCs infusion. Methods Diabetic infarcted animal models were created by streptozotocin (STZ, 40mg/Kg body weight) followed by intraperitoneal injection of isoproterenol in Wister rats (150 to 250 g). These animals received intravenous infusion of single and two consecutive doses of human umbilical cord blood (hUCB) derived MSCs. After seven days biochemical, glucose and insulin levels were quantified along with the pancreatic and cardiac markers transcript expression which were compared with the sham and control groups. Results Double dose infusion of hUCB‐MSCs in diabetic infarcted rats showed greater improvement compared to single dose in cardiovascular complications which were defined by Tropinin‐1, creatinine kinase, beta‐hydroxy butyrate and acetoacetate levels, cardiac transcript expression levels and histological analysis. While improvement in diabetic condition was observed by blood glucose and insulin levels and pancreatic transcription factors expression levels compared to sham and control group. Conclusion This study suggests that two consecutive doses of hUCB‐MSCs intravenous infusion exert comparatively better effects on the myocardial infarction and hyperglycemia in pre‐clinical settings.