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Human short peptidoglycan recognition protein PGLYRP1/Tag‐7/PGRP‐S inhibits Listeria monocytogenes intracellular survival in macrophages
Author(s) -
Slonova Darya,
Posvyatenko Alexandra,
Kibardin Alexey,
Ermolaeva Svetlana,
Severinov Konstantin,
Larin Sergey
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.01827
Subject(s) - listeria monocytogenes , microbiology and biotechnology , intracellular , intracellular parasite , biology , internalization , phagocytosis , peptidoglycan , proinflammatory cytokine , listeriolysin o , bacteria , macrophage , lipopolysaccharide , listeria , inflammation , biochemistry , immunology , cell , in vitro , genetics
PGLYRP1/Tag‐7/PGRP‐S is one of mammalian peptidoglycan recognition proteins (PGRPs). Here, we demonstrate that human recombinant PGLYRP1/Tag‐7/PGRP‐S potentiates the response of murine macrophage‐like ANA‐1 cells and human macrophages to facultative intracellular pathogen Listeria monocytogenes . PGLYRP1/ Tag‐7/PGRP‐S binds to the surface of L. monocytogenes and other bacterial cells but has no effect on their growth in culture. While PGLYRP1/Tag‐7/PGRP‐S treatment modestly enhanced phagocytosis of bacteria by ANA‐1 cells, the intracellular survival of PGLYRP1/ Tag‐7/PGRP‐S treated L. monocytogenes was strongly inhibited 2 h after internalization. PGLYRP1/Tag‐7/PGRP‐S treatment of bacteria boosted oxidative burst induction and increased the level of proinflammatory cytokine IL‐6 produced by ANA‐1, however, these effects happened too late to be responsible for decreased intracellular survival of bacteria. Our results thus suggest that PGLYRP1/Tag‐7/PGRP‐S acts as a molecular sensor for detection of L. monocytogenes infection of mammalian cells that leads to increased killing through a mechanism(s) that remains to be defined.