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Concentration of Immunoglobulins in Vaginal Secretion in Healthy Cows and with Mycoplasmosis
Author(s) -
Vasiliev Roman
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.01622
Subject(s) - antibody , vaginitis , vagina , radial immunodiffusion , physiology , mycoplasma , serology , biology , vaginal secretion , immunology , cervicitis , medicine , microbiology and biotechnology , gynecology , genetics
Inflammatory diseases of the genital tract have a significant effect on reducing the reproductive function of cows. These diseases cause significant economic damage to livestock farms in the Russian Federation. Among the many causative agents of infectious and inflammatory diseases of the genitals, representatives of the Mycoplasmataceae family occupy a special place, which is associated with the peculiarities of their biology. Therefore, the study of their influence on microecology and the protective properties of the vagina is an urgent task. The studies were carried out in JSC “Osminskoye” of the Slantsevsky district of the Leningrad region on non‐pregnant cows 3‐4 years old. Was formed 2 groups of 8 animals each. The first group is healthy cows in which the PCR test for Mycoplasma spp. was negative. The second group consisted of cows with a positive PCR test for Mycoplasma spp., Without pronounced clinical signs of vaginitis. M. bovigenitalium was identified by the serological method in these animals. In both groups of animals, vaginal secretions were collected from the vaginal wall using a special spoon in the cervical region. In the obtained secretion, the content of immunoglobulins of the classes Ig G, Ig M, Ig A and secretory immunoglobulin A (sIgA) was determined by the method of radial immunodiffusion in a gel according to Mancini. The results of the study show that the total content of immunoglobulins in vaginal secretions in healthy and mycoplasmosis animals was almost the same 0.32 g / l and 0.351 g / l, respectively. However, the distribution of immunoglobulins by class had significant differences. The Ig A concentration in cows with mycoplasmosis was 0.018 ± 0.001 g / l, which was 25% less than in healthy cows ‐ 0.024 ± 0.003 g / l, but the difference was not significant (P> 0.05). The level of Ig G in vaginal secretions in healthy cows and cows with mycoplasmosis was almost the same ‐ 0.19 ± 0.008 g / l and 0.183 ± 0.008 g / l. The content of immunoglobulin M in the vaginal secretion of healthy cows was 0.039 ± 0.002 g / l, while in patients with mycoplasmosis it was significantly higher ‐ 0.054 ± 0.003 g / l and the change was significant (P <0.01). Similar dynamics was observed for secretory immunoglobulin A (sIgA). In healthy cows, its sIgA level was 0.067 ± 0.005 g / l, while in patients with mycoplasmosis it was 43% higher ‐ 0.096 ± 0.007 g / l, and the differences had a high degree of reliability (P <0.001). The results of the study show that persistence of Mycoplasma bovigenitalium in the vagina of cows causes a local immune response, which is manifested by a tendency towards a decrease in Ig A, a significant increase in the levels of Ig M and sIgA, against the background of a stable content of Ig G.