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Antidiabetic and antioxidant effect of silver nanoparticles synthesized from the aqueous extract of Eryngium carlinae in the brain of streptozotocin‐induced diabetic rats
Author(s) -
Lemusde la Cruz Jenaro,
Landa Moreno Cinthia Itzel,
TrejoHurtado Cristian Mitchell,
PeñaMontes Donovan Javier,
HuertaCervantes Maribel,
ManzoAvalos Salvador,
SalgadoGarciglia Rafael,
MontoyaPérez Rocío,
CortésRojo Christian,
RosasTrejo Gerardo,
SaavedraMolina Alfredo
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.01490
Subject(s) - streptozotocin , antioxidant , aqueous extract , silver nanoparticle , chemistry , traditional medicine , pharmacology , aqueous solution , diabetes mellitus , nanoparticle , medicine , nanotechnology , endocrinology , materials science , organic chemistry
Diabetes mellitus (DM) is a metabolic disorder of multiple etiology that is characterized by chronic hyperglycemia and that affects more than 400 million people in the world, and with the progress over several years this can cause damage to different organs and different complications. One of the organs most affected by diabetes is the brain, which after development for several years can trigger cognitive decline, alterations in neurotransmission, and increased risk of Alzheimer's disease, among others. One of the fundamental mechanisms by which DM develops different complications is the high production of reactive oxygen species (ROS) in the mitochondria, which can induce a redox alteration since the antioxidant defense system is unable to neutralize the large amount of ROS.In the present study we investigated and compared the in vitro and in vivo antioxidant activity of the aqueous extract of Eryngium carlinae and silver nanoparticles (AgNP's) synthesized from the aqueous extract and silver nitrate (AgNO_3), as well as the hypoglycemic and hypolipidemic effect in vivo. The antioxidant activity in vitro was measured by the neutralizing activity of the 2,2‐diphenyl‐1‐picrylhydrazyl radical, the content of total phenols was also measured using the Folin‐Ciocalteau reagent for both the extract and the extract‐AgNP´s combination. Finally, we analyzed the activity of the extract and the extract‐AgNP's combination in serum and brain samples from diabetic (streptozotocin‐induced) and normoglycemic male Wistar rats. The extract‐AgNP's combination decreased blood glucose levels, while both the extract and the extract‐AgNP's combination decreased ROS production, and increased catalase activity only in normoglycemic groups. SOD in diabetic groups and in the activity of complexes of the electron transport chain. Our results suggest an antioxidant activity of the aqueous extract of E. carlinae and the extract‐AgNP's combination in the brain of diabetic rats, as well as hypoglycemic activity only in the extract‐AgNP's combination.