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Construct structurally defined Low molecular Heparan Sulfate microarray and its application
Author(s) -
Su Guowei
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.09797
Subject(s) - heparan sulfate , glycan , microarray analysis techniques , sulfation , microarray , chemistry , biochemistry , computational biology , biology , glycosaminoglycan , gene , glycoprotein , gene expression
Glycan microarray analysis is a high throughput method to screen the interaction between heparin oligosaccharides and target proteins. It is a powerful tool to investigate the binding between carbohydrates and carbohydrate‐binding proteins. Unlike other screening methods, microarray analysis uses a very small amount of target proteins (10 μg) to screen an entire library of Heparan Sulfate (HS) oligosaccharides. Currently we offers the microarray analysis covering up to 52 structurally defined HS oligosaccharides with a size range from pentasaccharides (5‐mer) to octodecasaccharides (18‐mer) representing many different sulfation types that can be found in nature. Support or Funding Information National Heart, Lung and Blood Institute, 1R01HL094463‐05National Institute of General Medical Sciences, 1U01GM1022137‐01, 1R41GM123792US FDA: U19FD004994‐01,NIH SBIR HHSN261201500019CSymbolic structures of low molecular heparan sulfate synthesized for microarrayWorkflow of Printed glycan arrays with fluorescent readout.